Journal: The Malaysian Journal of Medical Sciences : MJMS

Article Title: Development of DNA-Based Lateral Flow Assay for Detection of LDLR Gene Mutation for Familial Hypercholesterolemia

doi: 10.21315/mjms2024.31.3.6

Figure Lengend Snippet: sP and sN calculation

Article Snippet: Briefly, a panel assay was designed involving the primer design for the PCR process and the iPLEX reaction for the variant of interest (E101K) using the MassARRAY ® Assay Design Suite (ADS) software (Agena Bioscience, USA).

Techniques:

Journal: The Malaysian Journal of Medical Sciences : MJMS

Article Title: Development of DNA-Based Lateral Flow Assay for Detection of LDLR Gene Mutation for Familial Hypercholesterolemia

doi: 10.21315/mjms2024.31.3.6

Figure Lengend Snippet: MassARRAY ® spectrum analysis for gBlock gene fragment and representative clinical samples for E101K, LDLR gene. A) Wild-type gBlock: negative. B) Mutant gBlock: positive. C) Healthy control, HC 1: negative. D) FH 1: positive. The presence of one peak (red boxes) on the spectrum analysis indicates homozygous form, while the presence of two peaks (red boxes) on the spectrum analysis indicates heterozygous form

Article Snippet: Briefly, a panel assay was designed involving the primer design for the PCR process and the iPLEX reaction for the variant of interest (E101K) using the MassARRAY ® Assay Design Suite (ADS) software (Agena Bioscience, USA).

Techniques: Mutagenesis, Control

Journal: BioMed Research International

Article Title: Aberrant Hypermethylation of Aldehyde Dehydrogenase 2 Promoter Upstream Sequence in Rats with Experimental Myocardial Infarction

doi: 10.1155/2015/503692

Figure Lengend Snippet: Primer sequences, product length, and CpG units used for MassARRAY.

Article Snippet: Sequenom MassARRAY platform (MassARRAY) was used to examine the methylation levels of ALDH2 promoter upstream sequence.

Techniques:

Journal: BioMed Research International

Article Title: Aberrant Hypermethylation of Aldehyde Dehydrogenase 2 Promoter Upstream Sequence in Rats with Experimental Myocardial Infarction

doi: 10.1155/2015/503692

Figure Lengend Snippet: Upstream sequence of ALDH2 core promoter. (a) The chart is the analysis result of Methyl Primer Express; red pillars stand for CpG sites, and bar underlying highlights CpG high density region; (b) another analysis in the same region; blue dots represent valid CpG sites while red dots are invalid which cannot be detected in MassARRAY. Valid dots were numbered.

Article Snippet: Sequenom MassARRAY platform (MassARRAY) was used to examine the methylation levels of ALDH2 promoter upstream sequence.

Techniques: Sequencing

Journal: BioMed Research International

Article Title: Aberrant Hypermethylation of Aldehyde Dehydrogenase 2 Promoter Upstream Sequence in Rats with Experimental Myocardial Infarction

doi: 10.1155/2015/503692

Figure Lengend Snippet: Two more pairs of primer for verification of upstream sequence. (a) SQ1 Primer and SQ1 MassARRAY result. (b) SQ3 primer MassARRAY result. Both (a) and (b) are performed according to primer pattern, and CpG numbers are showed in . * P < 0.01 versus Sham.

Article Snippet: Sequenom MassARRAY platform (MassARRAY) was used to examine the methylation levels of ALDH2 promoter upstream sequence.

Techniques: Sequencing

Journal: BioMed Research International

Article Title: Aberrant Hypermethylation of Aldehyde Dehydrogenase 2 Promoter Upstream Sequence in Rats with Experimental Myocardial Infarction

doi: 10.1155/2015/503692

Figure Lengend Snippet: DNA methylation level of target upstream sequence and related proteins expression. (a) MassARRAY analysis result of target upstream sequence for DAC group and DAC + MI group. Sham group and MI-1-week group are chosen as comparative groups ( * P < 0.05). (b) Western blot of related proteins, including DNMT1 and ALDH2 ( * P < 0.05).

Article Snippet: Sequenom MassARRAY platform (MassARRAY) was used to examine the methylation levels of ALDH2 promoter upstream sequence.

Techniques: DNA Methylation Assay, Sequencing, Expressing, Western Blot

Journal:

Article Title: Arsenicals Produce Stable Progressive Changes in DNA Methylation Patterns that are Linked to Malignant Transformation of Immortalized Urothelial Cells

doi: 10.1016/j.taap.2009.08.019

Figure Lengend Snippet: MassARRAY confirms the arsenical-mediated differential DNA methylation detected by gene promoter microarray analysis. DNA methylation levels in parental UROtsa cells and each of the malignantly transformed variants (MSC12, MSC24, and MSC36, MSC52, and ASSC cells) were assessed in six differentially methylated gene promoter regions using Sequenom MassARRAY. The values presented show the overall percent CpG methylation for each promoter region analyzed.

Article Snippet: DNA methylation levels in parental UROtsa cells and each of the malignantly transformed variants (MSC12, MSC24, and MSC36, MSC52, and ASSC cells) were assessed in six differentially methylated gene promoter regions using Sequenom MassARRAY.

Techniques: DNA Methylation Assay, Microarray, Transformation Assay, Methylation, CpG Methylation Assay

Journal:

Article Title: Arsenicals Produce Stable Progressive Changes in DNA Methylation Patterns that are Linked to Malignant Transformation of Immortalized Urothelial Cells

doi: 10.1016/j.taap.2009.08.019

Figure Lengend Snippet: Altered DNA methylation patterns are stable after long term removal of arsenicals. Sequenom MassARRAY was used to analyze the DNA methylation status of each sample. (A) DNA methylation levels were determined for six differentially methylated gene promoter regions in parental UROtsa, URO-MSC24 and URO-MSC24 cells grown in the absence of MMA (III) for 3 or 6 months. (B) DNA methylation levels were determined for six differentially methylated gene promoter regions in parental UROtsa, URO-MSC52, and URO-MSC52 cells grown in the absence of MMA (III) for 3 or 6 months. The values presented show the overall percent CpG methylation for each promoter region analyzed.

Article Snippet: DNA methylation levels in parental UROtsa cells and each of the malignantly transformed variants (MSC12, MSC24, and MSC36, MSC52, and ASSC cells) were assessed in six differentially methylated gene promoter regions using Sequenom MassARRAY.

Techniques: DNA Methylation Assay, Methylation, CpG Methylation Assay